Bimake mouse direct pcr kit
WebThe Mouse Direct PCR Kit provides a fast preparation and PCR amplification that is specifically designed for mouse genotyping. The Buffer L and Protease Plus rapidly … WebMouse Direct PCR Kit (For Genotyping).....18 Contents. 1 2 Customer Reviews Fig a: After decades of qPCR research, we were used to the TRIzol method for mRNA isolation. ... Cell to cDNA Kit (Bimake) （Thermo Fisher Scientific) TRIzol Reagent Cat #: B25601，B25602 Cell/Tissue to cDNA Kit (Magnetic Beads) B25601 B25602 25 Rxn 100 Rxn USD 125
Bimake mouse direct pcr kit
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WebBimake.com provide best-in-class supplies and reagents commonly used in experimental laboratories for medical and biological research, including mycoplasma removal reagents, DNA transfection reagents, mouse tail … WebMar 31, 2024 · Quantitative Real-Time PCR (qRT-PCR) The total mRNA from NPCs and neuronal cultures was extracted with the RNeasy Plus Mini Kit (ref. 74034, Qiagen). cDNA was generated using the High Capacity cDNA Retro-Transcription Kit, according to the manufacturer’s instructions (Ref. 4368814, ThermoFisher).
WebMouse Direct PCR Kit (For Genotyping) B40015. Bimake. 500 ractions. EUR 490.8. Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the … WebIn addition, this protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells and it is a heparin-binding protein. Furthermore, FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Recombinant mouse FGF-9 is synthesized as a 208 a.a. precursor that contains a 3 a.a. signal sequence.
WebThe Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication. Additional information. Brand: bimake. WebDescription. Bimake™ 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to …
WebOct 1, 2024 · According to the manufacturer's protocol, DNA was isolated from toes by using a Mouse Direct PCR Kit (Bimake, USA) and amplified by using three sets of primers consisting of the following three sequences: 5′-CTTTGGTAACCATCTGTATGCTGGG-3′ (sense primer for both the wild type and Nrf2 −/− genotypes), 5′ …
WebDescription. Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody. With high loading of HA-tagged protein (more than 0.6 mg … phonyr comdataWebBimake mouse direct pcr kit Mouse Direct Pcr Kit, supplied by Bimake, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more how does a contact lens workWebDescription. The Mouse Direct PCR Kit provides a fast preparation and PCRamplification that is specifically designed for mouse genotyping. TheBuffer L and Protease Plus rapidly digest mouse tail, ear and toe to release intact genomic DNA that can be used directly as the templatefor PCR amplification.By using this kit, the digestion process only ... how does a consulting firm workWeb1 hour ago · Genomic DNA from the mouse tail was extracted by using the Mouse Direct PCR Kit (Bimake, TX, USA, #B40015) according to the manufacturer’s instructions. ... #8480) were used as primary antibodies. The following steps used biotin-streptavidin HRP detection kits (ZSGB-BIO, China, SP-9001, SP-9002) according to the manufacturer’s … phonylandWebMouse Direct PCR Kit对鼠尾、鼠趾、鼠耳都适用，55℃消化15min和30min的效果无显著性差异，对于大片段3Kb也能有效扩增。 注：其中片段500bp和3Kb左右的引物是根据小鼠GAPDH基因设计的；片段1Kb … phonytechs ulmWebThe Bimake Mouse Direct Pcr Kit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Direct pcr mouse. Other Bimake products are available in stock. how does a contingent beneficiary workWebJun 22, 2024 · 106 Genomic DNA was obtained by the Mouse Direct PCR Kit (Bimake, B40013, 107 Shanghai) from mouse tail. Ggct-/-mice were genotyped with the following 108 primers: 109 GGCT-KO-F: 5’-TGAGTCATAGATCTGACAGCAAGAG-3’ 110 GGCT-KO-R: 5’-ATAACCCCTGTGAACCATCATTCA-3’ 111 Predicted PCR product size for wild type … phonys