Hct116 puromycin
WebFind jobs, housing, goods and services, events, and connections to your local community in and around Atlanta, GA on Craigslist classifieds. WebOct 7, 2024 · A Colon CSC-enriched spheroids were transduced with sgRNAs targeting HMGCR or FDPS, followed by puromycin selection for 6 days. Knockout efficacy was …
Hct116 puromycin
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WebDec 22, 2005 · Transfected HCT116 cells were maintained in medium supplemented with 1 μg/mL puromycin (Invitrogen Life Technologies). Stable overexpression of c-FLIP was assessed by Western blot analysis. siRNA Transfections siRNAs were designed using the Invitrogen RNA interference designer tool 1 1 … WebApr 12, 2024 · The maximal tumor growth delays in HCT116- DNMT1+/+ model were observed at day 12 and day 18 by decitabine and aza-T-dCyd treatment, respectively. …
WebGibco® fetal bovine serum, puromycin, ... HCT116 and HT29 cells were cultured in growth medium (DMEM supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin) in a humidified atmosphere with 5% CO2 at 37 °C. Both malignant cell lines were stably transfected with shSep15 or a control construct WebApr 30, 2024 · To obtain cell lines stably overexpressing the lncRNA Ftx, HCT116 cells were transfected with Ftx and screened with puromycin (2 μg/mL), whereas HCT116 cells transfected with Ftx-NC were used as its mock control. The stable lncRNA Ftx-interfered or -overexpressing cell lines were validated by real-time PCR with the following primers …
WebOct 28, 2013 · HCT116 TCF4/Wnt cells were cultured in the presence of 50 μg ml −1 hygromycin B, and HCT116 TCF/LEF cells were cultured in the presence of 10 μg ml −1 puromycin. Primary colon cancer ... WebJan 10, 2024 · Following puromycin selection we identified single clones of DLD-1 and HCT116 cells displaying a green fluorescence, which were isolated and expanded as monoclonal cell lines (Figure 6A). To confirm correct CB integration at the AAVS1 locus we performed PCR-based genotyping as illustrated in Figure 6 B (strategy outlined in Figure …
WebGeneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishment. Explore all Gibco selection antibiotics
WebSPOLIATION OF EVIDENCE From the Georgia Bar Journal By Lee Wallace The Wallace Law Firm, L.L.C. 2170 Defoor Hills Rd. Atlanta, Georgia 30318 404-814-0465 lagu dugem tahun 2000anWebApr 1, 2024 · Stable transfection of HCT116 p53 +/+ and HCT116 p53 −/− cell lines (a kind gift from Dr. B. Vogelstein) were performed as described previously [17], Transfected cells were selected by culturing in medium containing 4 μg/ml of puromycin (Sigma-Aldrich), and single cell colonies were isolated. Immunoblots lagu dugem tiktok terbaruWebApr 1, 2024 · After 72 h of transfection, 2 mg/ml puromycin was added to the culture medium for HCT116 or RKO selection, respectively. ... A HCT116 cells in the siHOXC6 group were more sensitive to 5-Fu, ... lagu duet terbaruWebHCT116 cells have been widely used in colorectal cancer research. HCT116/GFP (CMV) stable cells express GFP under the control of the CMV promoter. ... Puromycin: Biosafety Level: BSL-2: Shipped: Dry ice : Storage: Liquid Nitrogen: Catalog Number Product Size Price Qty; SC-1001. HCT 116/GFP (CMV) stable cells. 2x10^6 cells: Price: Quantity. … lagu dugem terbaru 2022WebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This … jeep grand prixWebHCT116-hNIS-Neo/Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells were transduced with LV-hNIS-Neo (LV013) and LV-Fluc-P2A-Puro (LV012) and selected using G418 and puromycin. jeep grand nomade talcaWebDNR LBRU Rev 7-20-20 NOTIFICATION OF SALE, THEFT, RECOVERY, DESTRUCTION OR ABANDONMENT OR MOVED FROM STATE FOR A GA REGISTERED VESSEL … jeep grand laredo 2020