Phosphopantetheine binding
WebPresumably, the phosphopantetheinyl group of coenzyme A is transferred to FenB by the same mechanism and subsequently results in the binding of the [ 14 C]isoleucine to the enzyme. We found that approximately 13% of FenB expressed in E. coli bound to the amino acid. Biochemical characterization of recombinant FenB. Phosphopantetheine, also known as 4'-phosphopantetheine, is a prosthetic group of several acyl carrier proteins including the acyl carrier proteins (ACP) of fatty acid synthases, ACPs of polyketide synthases, the peptidyl carrier proteins (PCP), as well as aryl carrier proteins (ArCP) of nonribosomal peptide synthetases (NRPS). It is also present in formyltetrahydrofolate dehydrogenase.
Phosphopantetheine binding
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Webcytoskeleton and flagella extracellular region protein binding, modification* transmembrane transport cellulose binding calcium ion binding microtubule-based movement calcium channel complex NAD(P)H oxidase activity small GTPase signal cell regulation and signalling* proteolysis* positive regulation of GTPase activity membrane kinesin complex WebOct 5, 2007 · Together with docking simulations that identified a potential phosphopantetheine binding groove, the structural and functional studies reveal that the C9 specificity is a result of active site geometry and substrate ring constraints. The results lay the foundation for the design of novel aromatic polyketide natural products with different ...
WebJul 1, 2003 · Phosphopantetheine adenylyltransferase (PPAT) regulates the key penultimate step in the essential coenzyme A (CoA) biosynthetic pathway. PPAT catalyzes the reversible transfer of an adenylyl group from Mg2+:ATP to 4′-phosphopantetheine to form 3′-dephospho-CoA (dPCoA) and pyrophosphate. WebThe P5 region is cleaved, resulting in clusters containing only fragments which are attached by the P7 region. This ensures that all copies are sequenced in the same direction. The …
WebGene Ontology Term: phosphopantetheine binding. GO ID. GO:0031177. Aspect. Molecular Function. Description. Binding to phosphopantetheine, the vitamin pantetheine 4'- … WebMay 30, 2024 · To characterize the Mab PPAT ligand binding propensities, we determined crystal structures of Mab PPAT in complex with natural substrates ATP and 4′-phosphopantetheine (PhP). ATP occupies a positively charged pocket in the Mab PPAT substrate binding groove with the adenine ring making hydrogen-bond contacts with side …
http://amigo.geneontology.org/amigo/term/GO:0031177
WebMar 8, 2024 · cl09936: PP-binding Superfamily. Phosphopantetheine attachment site. A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group … high ground artinyaWebphosphopantetheine prosthetic group in the T domain to yield tyrosyl-S-NovH (Figure 2). It has been our premise that all subsequent modification steps leading to aminocoumarin … how i met your mother hangi platformdaWebMar 1, 2024 · It was evident that residues around the phosphopantetheine binding site and the C‐terminal end of helix II of apo‐LipD showed higher intensities from amide exchange experiments, indicating faster exchange (Supporting Information, Fig. S3). The intensities of these residues were suppressed by the attached prosthetic group in holo‐LipD. how i met your mother groove is in the heartWebSep 4, 2024 · phosphopantetheine binding pocket, away from the solvent channel, forming a hydrogen bond with . Val73, and interacting via water molecules (not shown) with Gly71, Val74 and Asn105 (Figure 2C). highground advisors dallas txWebPhosphopantetheine tethering is a posttranslational modification that takes place on the active site serine of carrier proteins 138 – ‘acyl carrier proteins (ACPs) and peptidyl … high ground airsoft houstonhigh ground airsoft pro shopWebUsing Escherichia coli LipB as a model enzyme, we show here that the octanoyl-transferase mainly recognizes the 4'-phosphopantetheine-tethered acyl-chain of its donor substrate and weakly binds the apo-acyl carrier protein. We demonstrate LipB can accept octanoate from its own ACP and noncognate ACPs, as well as C8-CoA. how i met your mother happy lily day